Female mice are more susceptible to the development of allergic airway inflammation than male mice

Clin Exp Allergy. 2005 Nov;35(11):1496-503. doi: 10.1111/j.1365-2222.2005.02362.x.


Background: In humans the prevalence of asthma is higher among females than among males after puberty. The reason for this phenomenon is not clear.

Objective: We tested the hypothesis that female mice are more susceptible to the development of allergic asthma than male mice and studied allergic immune responses in the lung.

Methods: We compared allergic airway inflammation, i.e. methacholine (MCh) responsiveness, serum IgE, and cytokines, and the number of the different leucocytes in lungs of male and female BALB/c mice, twice-sensitized to ovalbumin (OVA) and subsequently challenged with OVA (OVA-mice) or phosphate-buffered saline (PBS-mice) aerosols on days 24-26, 30, and 31.

Results: OVA challenge significantly increased MCh responsiveness, numbers of eosinophils, CD4(+) T cells, CD4(+)/CD25(+) T cells, B cells, and levels of Thelper (Th)2 cytokines, total, and OVA-specific IgE. There was, however, also an effect of gender, with female mice responding to OVA challenges with higher numbers of eosinophils, CD4(+) T cells, B cells, and levels of IL-4, IL-13, IFN-gamma, total, and OVA-specific IgE than male mice. In contrast, female PBS-mice had significantly lower percentages of regulatory CD4(+)/CD25(+) T cells than males (females 4.2+/-0.2% vs. males 5.3+/-0.1% of CD4(+) T cells, P<0.05).

Conclusion: Female mice develop a more pronounced type of allergic airway inflammation than male mice after OVA challenge. The reduced percentage of regulatory T cells in the lungs of female PBS-mice may indicate that the level of these cells in the lung during the sensitization phase is important for the development and/or progression of an allergic immune response after multiple OVA challenges.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Asthma / immunology*
  • Asthma / pathology
  • B-Lymphocytes / immunology
  • Bronchial Provocation Tests
  • Bronchoalveolar Lavage Fluid / immunology
  • CD4 Antigens / immunology
  • Chemokine CCL5 / analysis
  • Eosinophils / immunology
  • Female
  • Immunoglobulin E / analysis
  • Interleukins / analysis
  • Lung / immunology*
  • Lung / pathology
  • Male
  • Methacholine Chloride / immunology
  • Mice
  • Mice, Inbred BALB C
  • Ovalbumin / immunology
  • Receptors, Interleukin-2 / immunology
  • Sex Factors
  • T-Lymphocytes / immunology
  • Th2 Cells / immunology


  • CD4 Antigens
  • Chemokine CCL5
  • Interleukins
  • Receptors, Interleukin-2
  • Methacholine Chloride
  • Immunoglobulin E
  • Ovalbumin