Junctional adhesion molecules (JAM)-B and -C contribute to leukocyte extravasation to the skin and mediate cutaneous inflammation

J Invest Dermatol. 2005 Nov;125(5):969-76. doi: 10.1111/j.0022-202X.2005.23912.x.


Leukocyte extravasation is a finely tuned process, in which transmigration is the final step. Transmigration depends on molecules located at borders of endothelial cells; e.g., junctional adhesion molecules (JAM-A, -B and -C). In vivo blockade of JAM-A lead to decreased migration of monocytes into the skin. In contrast, the role of JAM-B and -C in development of cutaneous inflammation is unknown. We therefore elicited an allergic contact dermatitis in mice using 2,4-dinitro-1-fluorobenzene. RT-PCR and immunofluorescent staining of healthy skin revealed a constitutive JAM-B (66.4%+/-6.7% of all vessels) and -C expression (88.6+/-13.2%), which remained constant after induction of contact dermatitis. Functional studies, in which either JAM-B or -C neutralizing antibodies were injected into sensitized mice prior to allergen challenge showed a concentration-dependent reduction of the contact dermatitis. Decreased ear swelling was accompanied by reduction of leukocyte infiltration as analyzed by hematoxylin and eosin (H&E) histology and enzyme activity. Combined antibody treatment at doses of 1.25 mg per kg bodyweight lead to additive inhibition of allergic contact dermatitis, indicating that JAM-B and -C may have distinct functions. In conclusion, interactions with JAM-B and -C are essential for development of cutaneous inflammation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies / pharmacology
  • Cell Adhesion Molecules / antagonists & inhibitors
  • Cell Adhesion Molecules / metabolism
  • Cell Adhesion Molecules / physiology*
  • Dermatitis, Allergic Contact / immunology*
  • Dermatitis, Allergic Contact / metabolism
  • Endothelium, Vascular / immunology*
  • Immunoglobulins / metabolism
  • Immunoglobulins / physiology*
  • Leukocyte Rolling / drug effects
  • Leukocyte Rolling / immunology*
  • Leukocytes / immunology
  • Leukocytes / metabolism
  • Membrane Proteins / antagonists & inhibitors
  • Membrane Proteins / metabolism
  • Membrane Proteins / physiology*
  • Mice
  • Mice, Inbred C57BL
  • RNA, Messenger / analysis
  • RNA, Messenger / metabolism
  • Skin / immunology
  • Skin / metabolism


  • Antibodies
  • Cell Adhesion Molecules
  • Immunoglobulins
  • Jam2 protein, mouse
  • Jam3 protein, mouse
  • Membrane Proteins
  • RNA, Messenger