Structural studies of the core region of Aeromonas salmonicida subsp. salmonicida lipopolysaccharide

Carbohydr Res. 2006 Jan 16;341(1):109-17. doi: 10.1016/j.carres.2005.10.017. Epub 2005 Nov 17.


The core oligosaccharide structure of the in vivo derived rough phenotype of Aeromonas salmonicida subsp. salmonicida was investigated by a combination of compositional, methylation, CE-MS and one- and two-dimensional NMR analyses and established as the following: [carbohydrate: see text] where R=alpha-D-Galp-(1-->4)-beta-D-GalpNAc-(1--> or alpha-D-Galp-(1--> (approx. ratio 4:3). Comparative CE-MS analysis of A. salmonicida subsp. salmonicida core oligosaccharides from strains A449, 80204-1 and an in vivo rough isolate confirmed that the structure of the core oligosaccharide was conserved among different isolates of A. salmonicida.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aeromonas salmonicida / chemistry*
  • Carbohydrate Sequence
  • Electrophoresis, Capillary
  • Electrophoresis, Polyacrylamide Gel
  • Lipopolysaccharides / chemistry*
  • Mass Spectrometry
  • Methylation
  • Molecular Sequence Data
  • Nuclear Magnetic Resonance, Biomolecular


  • Lipopolysaccharides