Characterization of a human SWI2/SNF2 like protein hINO80: demonstration of catalytic and DNA binding activity

Biochem Biophys Res Commun. 2006 Jan 6;339(1):313-20. doi: 10.1016/j.bbrc.2005.10.206. Epub 2005 Nov 15.


The proteins belonging to SWI2/SNF2 family of DNA dependent ATPases are important members of the chromatin remodeling complexes that are implicated in epigenetic control of gene expression. We have identified a human gene with a putative DNA binding domain, which belongs to the INO80 subfamily of SWI2/SNF2 proteins. Here we report the cloning, expression, and functional activity of the domains from hINO80 gene both in terms of the DNA dependent ATPase as well as DNA binding activity. A differential expression of the various domains within this gene is detected in human tissues while a ubiquitous expression is detected in mice. The intranuclear localization is demonstrated using antibodies directed against the DBINO domain of hINO80.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / genetics
  • Adenosine Triphosphatases / metabolism*
  • Animals
  • Catalysis
  • Cell Line
  • Cell Nucleus / metabolism*
  • Cloning, Molecular
  • DNA Helicases / genetics
  • DNA Helicases / metabolism*
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Gene Expression Regulation
  • Humans
  • Mice
  • Organ Specificity
  • Protein Structure, Tertiary


  • DNA-Binding Proteins
  • Adenosine Triphosphatases
  • DNA Helicases