Association of CAT polymorphisms with catalase activity and exposure to environmental oxidative stimuli

Free Radic Res. 2005 Dec;39(12):1345-50. doi: 10.1080/10715760500306711.

Abstract

We tested the hypotheses that catalase activity is modified by CAT single nucleotide polymorphisms (SNPs) (-262;-844), and by their interactions with oxidant exposures (coal dusts, smoking), lymphotoxin alpha (LTA, NcoI) and tumor necrosis factor (TNF, -308) in 196 miners. Erythrocyte catalase, superoxide dismutase, and glutathione peroxidase activities were measured. The CAT -262 SNP was related to lower catalase activity (104, 87 and 72 k/g hemoglobin for CC, CT and TT, respectively, p < 0.0001). Regardless of CAT SNPs, the LTA NcoI but not the TNF-308 SNP was associated with catalase activity (p = 0.04 and p = 0.8). CAT -262 T carriers were less frequent in highly exposed miners (OR = 0.39 [0.20-0.78], p = 0.007). In CAT -262 T carriers only, catalase activity decreased with high dust exposure (p = 0.01). Haplotype analyses (combined CAT SNPs) confirm these results. Results show that CAT -262 and LTA NcoI SNPs, and interaction with coal dust exposure, influenced catalase activity.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Catalase / blood
  • Catalase / genetics*
  • Catalase / metabolism*
  • Coal / toxicity*
  • Coal Mining
  • Dust
  • Environmental Pollutants / toxicity*
  • Erythrocytes / enzymology
  • Genotype
  • Glutathione Peroxidase / blood
  • Humans
  • Lymphotoxin-alpha / physiology
  • Middle Aged
  • Oxidants / toxicity*
  • Oxidation-Reduction
  • Polymorphism, Single Nucleotide
  • Smoke / adverse effects*
  • Superoxide Dismutase / blood
  • Tobacco / toxicity*
  • Tumor Necrosis Factor-alpha / physiology

Substances

  • Coal
  • Dust
  • Environmental Pollutants
  • Lymphotoxin-alpha
  • Oxidants
  • Smoke
  • Tumor Necrosis Factor-alpha
  • Catalase
  • Glutathione Peroxidase
  • Superoxide Dismutase