Positional proteomics: selective recovery and analysis of N-terminal proteolytic peptides

Nat Methods. 2005 Dec;2(12):955-7. doi: 10.1038/nmeth811. Epub 2005 Nov 18.


Bottom-up proteomics is the analysis of peptides derived from single proteins or protein mixtures, and because each protein generates tens of peptides, there is scope for controlled reduction in complexity. We report here a new strategy for selective isolation of the N-terminal peptides of a protein mixture, yielding positionally defined peptides. The method is tolerant of several fragmentation methods, and the databases that must be searched are substantially less complex.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biotinylation
  • Mass Spectrometry
  • Mice
  • Muscle Proteins / chemistry
  • Muscle Proteins / isolation & purification
  • Muscle, Skeletal / chemistry
  • Peptide Fragments / chemistry*
  • Peptide Fragments / isolation & purification
  • Peptide Mapping
  • Proteins / chemistry*
  • Proteins / isolation & purification
  • Proteomics / methods*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*


  • Muscle Proteins
  • Peptide Fragments
  • Proteins