Loss of polycystin-1 or polycystin-2 results in dysregulated apolipoprotein expression in murine tissues via alterations in nuclear hormone receptors

Hum Mol Genet. 2006 Jan 1;15(1):11-21. doi: 10.1093/hmg/ddi421. Epub 2005 Nov 21.


Autosomal dominant polycystic kidney disease (ADPKD) is caused by mutations of PKD1 and PKD2. Murine gene targeting studies have shown that these genes play an essential role in development, with homozygous inactivation resulting in embryonic lethality. Recently, Pkd1-/- lethality has been linked to placental insufficiency. In this study, the placenta was used as a model to identify factors involved in these developmental abnormalities. Microarray analysis of Pkd1-/- placentae showed upregulation of a set of apolipoprotein-related genes. These changes were validated and were found to be associated with increased quantities of apolipoproteins in the amniotic fluid. Increased apolipoprotein gene expression was also observed in Pkd2-/-placentae and in cystic kidneys of Pkd1cond/-; Meox2cre/+ mice. Using chromatin immunoprecipitation assays, we determined that the activity of HNF-4alpha, a major regulator of apolipoprotein gene expression, was also increased in these organs. These findings suggest a potential role for dysregulation of nuclear hormone receptors in the pathogenesis of ADPKD.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amniotic Fluid / metabolism
  • Animals
  • Apolipoproteins / metabolism*
  • Blotting, Northern
  • Blotting, Western
  • Chromatin Immunoprecipitation
  • DNA Primers
  • Female
  • Gene Expression Regulation / genetics*
  • Hepatocyte Nuclear Factor 4 / metabolism
  • Immunohistochemistry
  • Kidney / metabolism
  • Mice
  • Mice, Knockout
  • Microarray Analysis
  • Placenta / metabolism*
  • Polycystic Kidney, Autosomal Dominant / genetics*
  • Pregnancy
  • TRPP Cation Channels / metabolism*


  • Apolipoproteins
  • DNA Primers
  • Hepatocyte Nuclear Factor 4
  • TRPP Cation Channels
  • polycystic kidney disease 1 protein
  • polycystic kidney disease 2 protein

Associated data

  • GEO/GSM51337
  • GEO/GSM51338
  • GEO/GSM51339
  • GEO/GSM51340
  • GEO/GSM51341
  • GEO/GSM51342
  • GEO/GSM51343
  • GEO/GSM51344
  • GEO/GSM51345
  • GEO/GSM51346
  • GEO/GSM51347
  • GEO/GSM51348
  • GEO/GSM51349
  • GEO/GSM51350
  • GEO/GSM51351
  • GEO/GSM51352
  • GEO/GSM51353