Isolation and expression pattern of RGS21 gene, a novel RGS member

Acta Biochim Pol. 2005;52(4):943-6. Epub 2005 Sep 8.


Regulators of G-protein signaling (RGS) proteins are known for the RGS domain that is composed of a conserved stretch of 120 amino acids, which binds directly to activated G-protein alpha subunits and acts as a GTPase-activating protein (GAP), leading to their deactivation and termination of downstream signals. In this study, a novel human RGS cDNA (RGS21), 1795 bp long and encoding a 152-amino acid polypeptide, was isolated by large-scale sequencing analysis of a human fetal brain cDNA library. Unlike other RGS family members, RGS21 gene has no additional domain/motif and may represent the smallest known member of RGS family. It may belong to the B/R4 subfamily, which suggests that it may serve exclusively as a negative regulator of alphai/o family members and/or alphaq/11. PCR analysis showed that RGS21 mRNA was expressed ubiquitously in the 16 tissues examined, implying general physiological roles.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • DNA, Complementary
  • GTP-Binding Protein Regulators / genetics*
  • Gene Expression Regulation*
  • Humans
  • Molecular Sequence Data
  • Phylogeny
  • RGS Proteins
  • RNA, Messenger / genetics
  • Restriction Mapping
  • Sequence Alignment


  • DNA, Complementary
  • GTP-Binding Protein Regulators
  • RGS Proteins
  • RGS21 protein, human
  • RNA, Messenger

Associated data

  • GENBANK/AY643711