Preferential migration of effector CD8+ T cells into the interstitium of the normal lung

J Clin Invest. 2005 Dec;115(12):3473-83. doi: 10.1172/JCI24482. Epub 2005 Nov 23.


The respiratory tract is a primary site of infection and exposure to environmental antigens and an important site of memory T cell localization. We analyzed the migration and retention of naive and activated CD8+ T cells within the noninflamed lungs and quantitated the partitioning of adoptively transferred T cells between the pulmonary vascular and interstitial compartments. Activated but not naive T cells were retained within the lungs for a prolonged period. Effector CD8+ T cells preferentially egressed from the pulmonary vascular compartment into the noninflamed pulmonary interstitium. T cell retention within the lung vasculature was leukocyte function antigen-1 dependent, while the egress of effector T cells from the vascular to the interstitium functions through a pertussis toxin-sensitive (PTX-sensitive) mechanism driven in part by constitutive CC chemokine ligand 5 expression in the lungs. These results document a novel mechanism of adhesion receptor- and pulmonary chemokine-dependent regulation of the migration of activated CD8+ T cells into an important nonlymphoid peripheral site (i.e., the normal/noninflamed lung).

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CD8-Positive T-Lymphocytes / immunology*
  • CD8-Positive T-Lymphocytes / metabolism
  • Cell Adhesion Molecules / metabolism
  • Cell Movement*
  • Cell Separation
  • Chemokine CCL5 / metabolism
  • Chemokines / metabolism
  • Flow Cytometry
  • Immunologic Memory
  • Inflammation
  • Lung / cytology*
  • Lung / pathology*
  • Lymphocyte Activation
  • Lymphocyte Function-Associated Antigen-1 / metabolism
  • Mice
  • Mice, Inbred BALB C
  • Mice, Transgenic
  • Pertussis Toxin / pharmacology
  • Respiratory System / pathology
  • T-Lymphocytes / immunology*
  • T-Lymphocytes / metabolism
  • T-Lymphocytes, Regulatory / immunology
  • T-Lymphocytes, Regulatory / metabolism
  • Time Factors


  • Cell Adhesion Molecules
  • Chemokine CCL5
  • Chemokines
  • Lymphocyte Function-Associated Antigen-1
  • Pertussis Toxin