Global synthetic-lethality analysis and yeast functional profiling

Trends Genet. 2006 Jan;22(1):56-63. doi: 10.1016/j.tig.2005.11.003. Epub 2005 Nov 23.


The Saccharomyces genome-deletion project created >5900 'molecularly barcoded' yeast knockout mutants (YKO mutants). The YKO mutant collections have facilitated large-scale analyses of a multitude of mutant phenotypes. For example, both synthetic genetic array (SGA) and synthetic-lethality analysis by microarray (SLAM) methods have been used for synthetic-lethality screens. Global analysis of synthetic lethality promises to identify cellular pathways that 'buffer' each other biologically. The combination of global synthetic-lethality analysis, together with global protein-protein interaction analyses, mRNA expression profiling and functional profiling will, in principle, enable construction of a cellular 'wiring diagram' that will help frame a deeper understanding of human biology and disease.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Chromosome Mapping
  • Gene Deletion
  • Gene Expression Profiling
  • Genes, Fungal
  • Genes, Lethal
  • Genetic Techniques
  • Genome, Fungal
  • Oligonucleotide Array Sequence Analysis
  • RNA, Fungal / genetics
  • RNA, Messenger / genetics
  • Saccharomyces cerevisiae / genetics*


  • RNA, Fungal
  • RNA, Messenger