Detection and quantification of ginsenoside Re in ginseng samples by a chromatographic immunostaining method using monoclonal antibody against ginsenoside Re

Osamu Morinaga; Hiroyuki Tanaka; Yukihiro Shoyama

doi:10.1016/j.jchromb.2005.10.040

Detection and quantification of ginsenoside Re in ginseng samples by a chromatographic immunostaining method using monoclonal antibody against ginsenoside Re

J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Jan 2;830(1):100-4. doi: 10.1016/j.jchromb.2005.10.040. Epub 2005 Nov 28.

Authors

Osamu Morinaga¹, Hiroyuki Tanaka, Yukihiro Shoyama

Affiliation

¹ Innovation Plaza Fukuoka, Japan Science and Technology Agency, Sawara-ku, Fukuoka.

PMID: 16310421
DOI: 10.1016/j.jchromb.2005.10.040

Abstract

A chromatographic immunostaining method has been developed for the determination of ginsenoside Re (G-Re) in ginseng samples on a polyethersulphone (PES) membrane. G-Re standard and the extracts of ginseng roots were applied to a PES membrane and developed by methanol-water-acetic acid (45:55:1, by volume). G-Re was clearly detected by an immunostaining method using a monoclonal antibody against G-Re. The coloring spots of G-Re were analyzed quantitatively using NIH Image software indicating at least 0.125 microg of G-Re was detectable. G-Re can be analyzed quantitatively between 0.25 and 4.0 microg.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Monoclonal / immunology*
Chromatography, Thin Layer / methods*
Female
Ginsenosides / analysis*
Ginsenosides / immunology
Mice
Mice, Inbred BALB C
Nucleic Acid Hybridization
Panax / chemistry*
Reproducibility of Results

Substances

Antibodies, Monoclonal
Ginsenosides
ginsenoside Re