Localization of the nuclear receptor CAR at the cell membrane of mouse liver

FEBS Lett. 2005 Dec 19;579(30):6733-6. doi: 10.1016/j.febslet.2005.10.070. Epub 2005 Nov 21.

Abstract

The nuclear receptor constitutive active/androstane receptor CAR is a drug-sensing transcription factor. Upon activation by various drugs such as phenobarbital (PB), CAR translocates from the cytoplasm into the nucleus to regulate the genes that encode enzymes and proteins involved in hepatic metabolism. Here, we have shown the presence of CAR at the cell membrane of mouse livers, using Car+/+ and Car-/- mice. Levels of the cell membrane CAR increased after PB treatment. The CAR exists as a large approximately 160 kDa complex. Thus, CAR undergoes PB-induced translocation to the cell membrane, indicating that CAR may exert a non-genomic action.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural

MeSH terms

  • Animals
  • Cell Membrane / metabolism*
  • Cell Nucleus / metabolism
  • Liver / cytology*
  • Liver / drug effects
  • Liver / metabolism*
  • Liver Extracts / chemistry
  • Liver Extracts / metabolism
  • Mice
  • Mice, Inbred Strains
  • Mice, Knockout
  • Molecular Weight
  • Phenobarbital / pharmacology
  • Receptors, Cytoplasmic and Nuclear / analysis
  • Receptors, Cytoplasmic and Nuclear / genetics
  • Receptors, Cytoplasmic and Nuclear / metabolism*
  • Subcellular Fractions / chemistry
  • Time Factors
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*

Substances

  • Liver Extracts
  • Receptors, Cytoplasmic and Nuclear
  • Transcription Factors
  • Phenobarbital