A role for LEDGF/p75 in targeting HIV DNA integration

Nat Med. 2005 Dec;11(12):1287-9. doi: 10.1038/nm1329. Epub 2005 Nov 27.


HIV DNA integration is favored in active genes, but the underlying mechanism is unclear. Cellular lens epithelium-derived growth factor (LEDGF/p75) binds both chromosomal DNA and HIV integrase, and might therefore direct integration by a tethering interaction. We analyzed HIV integration in cells depleted for LEDGF/p75, and found that integration was (i) less frequent in transcription units, (ii) less frequent in genes regulated by LEDGF/p75 and (iii) more frequent in GC-rich DNA. LEDGF is thus the first example of a cellular protein controlling the location of HIV integration in human cells.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing / genetics
  • Adaptor Proteins, Signal Transducing / metabolism*
  • Blotting, Western
  • Cell Line
  • DNA / metabolism*
  • Gene Silencing
  • HIV / genetics*
  • HIV / physiology
  • HIV Integrase / metabolism
  • Humans
  • Microarray Analysis
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Virus Integration / physiology*


  • Adaptor Proteins, Signal Transducing
  • PSIP1 protein, human
  • Transcription Factors
  • DNA
  • HIV Integrase