Analysis of macamides in samples of Maca (Lepidium meyenii) by HPLC-UV-MS/MS

Phytochem Anal. 2005 Nov-Dec;16(6):463-9. doi: 10.1002/pca.871.


The macamides are a distinct class of secondary metabolites that have so far been found only in Lepidium meyenii Walp. (Maca). Using HPLC-UV-MS/MS, the main macamides have been identified as n-benzylhexadecanamide, n-benzyl-(9Z)-octadecenamide, n-benzyl-(9Z, 12Z)-octadecadienamide, n-benzyl-(9Z, 12Z, 15Z)-octadecatrienamide and n-benzyloctadecanamide. The identities of n-benzyl-(9Z)-octadecenamide and n-benzyl-(9Z, 12Z)-octadecadienamide were confirmed by comparison of chromatographic and spectral properties with synthetic analogues. Total macamides have been quantified by HPLC-UV in plant material from different vendors using n-benzylhexadecanamide as an external standard. The amount of macamides in the dried plant material ranged from 0.0016 to 0.0123%.

MeSH terms

  • Amides / analysis*
  • Amides / chemistry*
  • Chromatography, High Pressure Liquid
  • Lepidium / chemistry*
  • Mass Spectrometry
  • Molecular Structure
  • Spectrophotometry, Ultraviolet


  • Amides