An alpha/beta-fold C--C Bond Hydrolase Is Involved in a Central Step of Nicotine Catabolism by Arthrobacter Nicotinovorans

J Bacteriol. 2005 Dec;187(24):8516-9. doi: 10.1128/JB.187.24.8516-8519.2005.

Abstract

The enzyme catalyzing the hydrolytic cleavage of 2,6-dihydroxypseudooxynicotine to 2,6-dihydroxypyridine and gamma-N-methylaminobutyrate was found to be encoded on pAO1 of Arthrobacter nicotinovorans. The new enzyme answers an old question about nicotine catabolism and may be the first C--C bond hydrolase that is involved in the biodegradation of a heterocyclic compound.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaloids / metabolism
  • Arthrobacter / enzymology*
  • Arthrobacter / metabolism
  • Biodegradation, Environmental
  • Chromatography, High Pressure Liquid
  • Cloning, Molecular
  • Gene Order
  • Heterocyclic Compounds / metabolism
  • Hydrolases / chemistry*
  • Hydrolases / metabolism*
  • Nicotine / analogs & derivatives
  • Nicotine / metabolism*
  • Open Reading Frames
  • Plasmids / genetics
  • Pyridines / metabolism

Substances

  • Alkaloids
  • Heterocyclic Compounds
  • Pyridines
  • pseudonicotine
  • 2,6-dihydroxypyridine
  • Nicotine
  • Hydrolases