Phenotype and function of myeloid dendritic cells derived from African green monkey blood monocytes

J Immunol Methods. 2006 Jan 20;308(1-2):138-55. doi: 10.1016/j.jim.2005.10.005. Epub 2005 Nov 21.


Myeloid dendritic cells probably play an important role in the immune response against HIV and SIV, and in the enhancement of CD4+ T cell infection. Here, we have investigated phenotypic and functional features of myeloid monocyte-derived DC (MDDC) from African green monkeys (AGMs). AGMs are natural hosts of SIV and exhibit no signs of abnormal T cell activation despite high SIV plasma viremia. We identified mAbs that cross-react specifically with homologous molecules expressed on AGM DC. We adapted a protocol to derive AGM MDDC by culture in the presence of GM-CSF and IL-4. The differentiated cells possessed a typical dendritic morphology and the majority were CD11c+ DC-SIGN+. AGM MDDC displayed a high expression of typical maturation markers, such as CD83, CD86 and DC-LAMP, and moderate immunostimulatory capacity, suggesting that the cells were in a semi-mature state. Stimulation resulted in further maturation, as shown by up-regulation of CD80 and decrease of endocytosis ability. However, neither increase of HLA-DR or CD40 expression nor enhanced immunostimulatory capacity was observed. The latter was associated with a low pro-inflammatory cytokine production during mixed lymphocyte reactions and a cytokine balance in favour of IL-10 in contrast to human MDDC. This is the first characterization of AGM MDDC. The tools described here are a crucial step for future studies in vivo or in vitro on the function of myeloid DC using the AGM animal model.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cell Differentiation
  • Cell Separation
  • Chlorocebus aethiops / blood*
  • Chlorocebus aethiops / genetics
  • Chlorocebus aethiops / immunology*
  • Cytokines / biosynthesis
  • Cytokines / genetics
  • DNA / genetics
  • Dendritic Cells / cytology
  • Dendritic Cells / immunology*
  • Female
  • Flow Cytometry
  • Gene Expression
  • Humans
  • Immunophenotyping
  • In Vitro Techniques
  • Interferon-gamma / genetics
  • Interleukin-10 / biosynthesis
  • Interleukin-10 / genetics
  • Interleukin-12 / biosynthesis
  • Interleukin-12 / genetics
  • Lymphocyte Culture Test, Mixed
  • Male
  • Models, Animal
  • Monocytes / cytology
  • Monocytes / immunology*
  • Myeloid Cells / cytology
  • Myeloid Cells / immunology*
  • Phagocytosis
  • Tumor Necrosis Factor-alpha / genetics


  • Cytokines
  • Tumor Necrosis Factor-alpha
  • Interleukin-10
  • Interleukin-12
  • Interferon-gamma
  • DNA