Protective effect of Acorus calamus LINN on free radical scavengers and lipid peroxidation in discrete regions of brain against noise stress exposed rat

Biol Pharm Bull. 2005 Dec;28(12):2327-30. doi: 10.1248/bpb.28.2327.


Exposure to continuous loud noise is a serious health problem due to excess production of oxygen free radicals. In medical research, more attention is paid to the antioxidant properties of medicinal plants to minimize the harmful effects of radicals. The aim of this study was to evaluate the protective effect of both ethyl acetate and methanolic extract of Acorus calamus LINN against noise stress (30 d, 100 dBA/4h/d) induced changes in the rat brain. We measured the activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and the levels of reduced glutathione (GSH), vitamin C, vitamin E, protein thiols and lipid peroxidation (LPO) for the evaluation of oxidative stress status in discrete regions of the rat brain like cerebral cortex, cerebellum, pons-medulla, midbrain, hippocampus and hypothalamus. The results indicated that during exposure of noisy environment ROS generation led to increase in corticosterone, LPO and SOD, but decrease in CAT, GPx, GSH, protein thiols, vitamins C and E levels. Both the ethyl acetate and methanolic extract of Acorus calamus protected most of the changes in the rat brain induced by noise-stress.

MeSH terms

  • Acetates / pharmacology
  • Acorus / chemistry*
  • Animals
  • Ascorbic Acid / metabolism
  • Brain / drug effects*
  • Brain / enzymology
  • Brain / metabolism*
  • Catalase / drug effects
  • Catalase / metabolism
  • Drug Evaluation, Preclinical
  • Free Radical Scavengers / chemistry
  • Free Radical Scavengers / pharmacology*
  • Glutathione / drug effects
  • Glutathione / metabolism
  • Glutathione Peroxidase / drug effects
  • Glutathione Peroxidase / metabolism
  • Lipid Peroxidation / drug effects*
  • Male
  • Methanol / pharmacology
  • Noise / adverse effects*
  • Plant Extracts / pharmacology
  • Rats
  • Rats, Wistar
  • Rhizome / chemistry
  • Stress, Psychological / enzymology
  • Stress, Psychological / etiology
  • Stress, Psychological / metabolism*
  • Sulfhydryl Compounds / metabolism
  • Superoxide Dismutase / drug effects
  • Superoxide Dismutase / metabolism
  • Vitamin E / metabolism


  • Acetates
  • Free Radical Scavengers
  • Plant Extracts
  • Sulfhydryl Compounds
  • Vitamin E
  • ethyl acetate
  • Catalase
  • Glutathione Peroxidase
  • Superoxide Dismutase
  • Glutathione
  • Ascorbic Acid
  • Methanol