Volatile anesthetics selectively alter [3H]ryanodine binding to skeletal and cardiac ryanodine receptors

Biochem Biophys Res Commun. 1992 Jul 15;186(1):595-600. doi: 10.1016/s0006-291x(05)80850-2.


The effect of clinical concentrations of volatile anesthetics on ryanodine receptors of cardiac and skeletal muscle sarcoplasmic reticulum was evaluated using [3H]ryanodine binding. At 2 volume percent, halothane and enflurane stimulated binding to cardiac SR by 238% and 204%, respectively, while isoflurane had no effect. In contrast, halothane and enflurane had no effect on [3H]ryanodine binding to skeletal ryanodine receptors, while isoflurane produced a significant stimulation. These results suggest that volatile anesthetics interact in a site-specific manner with ryanodine receptors of cardiac or skeletal muscle to effect Ca2+ release-channel gating.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Calcium / pharmacology
  • Dose-Response Relationship, Drug
  • Enflurane / pharmacology*
  • Halothane / pharmacology*
  • Isoflurane / pharmacology*
  • Kinetics
  • Muscles / metabolism*
  • Myocardium / metabolism*
  • Receptors, Cholinergic / drug effects
  • Receptors, Cholinergic / metabolism*
  • Ryanodine / metabolism*
  • Ryanodine Receptor Calcium Release Channel
  • Sarcoplasmic Reticulum / metabolism
  • Swine
  • Tritium


  • Receptors, Cholinergic
  • Ryanodine Receptor Calcium Release Channel
  • Tritium
  • Ryanodine
  • Enflurane
  • Isoflurane
  • Calcium
  • Halothane