Fermentation of 10% (w/v) sugar to D: (-)-lactate by engineered Escherichia coli B

Biotechnol Lett. 2005 Dec;27(23-24):1891-6. doi: 10.1007/s10529-005-3899-7.

Abstract

Derivatives of ethanologenic Escherichia coli K011 were constructed for D: (-)-lactate production by deleting genes encoding competing pathways followed by metabolic evolution, a growth-based selection for mutants with improved performance. Resulting strains, SZ132 and SZ186, contain native genes for sucrose utilization. No foreign genes are present in SZ186. Strain SZ132 also contains a chromosomally integrated endoglucanase gene (Erwinia chrysanthemi celY). Strain SZ132 produced over 1 mol lactate per liter of complex medium containing 10% (w/v) sugar (fermentation times of 48 h for glucose, 120 h for sucrose). Both strains produced 667-700 mmol lactate per liter of mineral salts medium. Yields for metabolized sugar ranged from 88% to 95% in both media.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Carboxylic Acids / metabolism
  • Culture Media / pharmacology
  • Escherichia coli / drug effects
  • Escherichia coli / genetics*
  • Escherichia coli / metabolism
  • Fermentation / genetics
  • Gene Deletion
  • Genetic Engineering / methods
  • Glucose / metabolism
  • Lactic Acid / biosynthesis*
  • Lactic Acid / chemistry
  • Mutation / genetics
  • Stereoisomerism
  • Sucrose / metabolism*
  • Transduction, Genetic

Substances

  • Carboxylic Acids
  • Culture Media
  • Lactic Acid
  • Sucrose
  • Glucose