[Study on the frequency of alpha-1,2-fucosyltransferase gene h4 allele (C35T) in Chinese population]

Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2005 Dec;22(6):657-60.
[Article in Chinese]


Objective: To investigate the h4 allele (C35T) frequency of alpha-1,2-fucosyltransferase gene in Chinese population.

Methods: The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for identifying C35T variant was established by using PCR to amplify a 125 bp FUT1 gene fragment, including C35T variant sequence, and the PCR product was digested by Hae III restriction enzyme. One hundred and fifty-eight random samples from Chinese blood donor were screened by PCR-RFLP.

Results: Among 158 Chinese individuals with normal ABO and H blood group phenotypes, 8 and 83 were homozygous with 35T/T and 35C/C, respectively, while 67 were heterozygous with 35C/T. The allele frequencies were compatible with Hardy-Weinberg equilibrium.

Conclusion: The C35T substitution of FUT1 gene is not a mutation which gives rise to a non-functional h allele responsible for para-Bombay phenotype but a single nucleotide polymorphism in Chinese population.

Publication types

  • English Abstract

MeSH terms

  • ABO Blood-Group System / genetics*
  • Alleles
  • Female
  • Fucosyltransferases / genetics*
  • Gene Frequency
  • Genotype
  • Humans
  • Male
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length


  • ABO Blood-Group System
  • Fucosyltransferases
  • galactoside 2-alpha-L-fucosyltransferase