[Telomerase activity and telomere length in CD4+,CD8+ and CD19+ lymphocytes from patients with systemic lupus erythematosus]

Zhejiang Da Xue Xue Bao Yi Xue Ban. 2005 Nov;34(6):534-7.
[Article in Chinese]

Abstract

Objective: To investigate the telomerase activity and the telomere length in CD4(+), CD8(+) and CD19(+) lymphocytes from patients with systemic lupus erythematosus (SLE).

Methods: Telomerase activity of CD4(+), CD8(+) and CD19(+) cells from patients with SLE and normal controls was examined by telomeric repeats amplification protocol. Telomere length in those cells was measured by Southern blot method.

Result: CD4(+), CD8(+) and CD19(+) cells in patients with SLE showed high telomerase activity, but only telomerase activity of CD19(+) cells was significantly higher than that in controls. The length of telomere in CD4(+) and CD8(+) cells was significantly shorter than that in controls, but not in CD19(+) cells.

Conclusion: Higher telomerase activity in CD19(+) cells and shortened telomere length in CD4(+) and CD8(+) cells of patients with SLE may be associated with pathogenesis of SLE.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Antigens, CD19 / immunology
  • CD4-Positive T-Lymphocytes / enzymology*
  • CD8-Positive T-Lymphocytes / enzymology*
  • Female
  • Humans
  • Lupus Erythematosus, Systemic / enzymology
  • Lupus Erythematosus, Systemic / immunology*
  • Lymphocytes / enzymology
  • Male
  • Middle Aged
  • Telomerase / metabolism*
  • Telomere / genetics*

Substances

  • Antigens, CD19
  • Telomerase