Establishment and characterization of a new erythroblastic leukemia cell line, EEB: phosphatidylglucoside-mediated erythroid differentiation and apoptosis

Leuk Res. 2006 Jul;30(7):829-39. doi: 10.1016/j.leukres.2005.10.021. Epub 2005 Dec 5.


A new erythroblastic leukemia cell line (EEB) was established from a patient with early erythroblastic leukemia. The cells had features of immature erythroblasts, including an agranular basophilic cytoplasm and CD36, CD71, CD175s (sialyl-Tn) and CD235a (glycophorin A) expression without CD41 expression, myeloperoxidase activity and platelet-peroxidase activity. The cells were confirmed to be of the erythroid lineage based on expression of the gamma-globin message. They were induced to differentiate into benzidine-positive cells by hemin and delta-amino levulinic acid (delta-ALA). An analysis of cell membrane lipids showed that EEB cells contain a type of glycerolipid, phosphatidylglucose (PhGlc), but not unbranched type 2 chains, i antigens. GL-7 which is a recombinant Fab fragment of GL-2 and binds to PhGlc, induced production of hemoglobin F (HbF) associated with accumulation of the gamma-globin (gamma-globin) message in EEB cells. The GL-7-mediated erythroid differentiation was associated with apoptosis. These results suggest that direct signaling to PhGlc mediates erythroid differentiation and apoptosis in EEB cells.

Publication types

  • Case Reports

MeSH terms

  • Apoptosis / drug effects*
  • Cell Differentiation / drug effects
  • Cell Line, Tumor*
  • Cytogenetic Analysis
  • Drug Screening Assays, Antitumor
  • Erythroid Cells / cytology
  • Erythroid Cells / drug effects*
  • Globins / drug effects
  • Globins / genetics
  • Glycerophospholipids / analysis
  • Glycerophospholipids / pharmacology*
  • Humans
  • Immunoglobulin Fab Fragments / pharmacology*
  • Immunophenotyping
  • Leukemia, Erythroblastic, Acute / drug therapy*
  • Leukemia, Erythroblastic, Acute / genetics
  • Leukemia, Erythroblastic, Acute / metabolism*
  • Male
  • Middle Aged
  • Phosphorylation / drug effects
  • Reverse Transcriptase Polymerase Chain Reaction / methods
  • Sensitivity and Specificity
  • Structure-Activity Relationship
  • Tyrosine / drug effects
  • Tyrosine / metabolism


  • GL-7 Fab fragment
  • Glycerophospholipids
  • Immunoglobulin Fab Fragments
  • phosphatidylglucose
  • Tyrosine
  • Globins