Cannabinoid receptor ligands mediate growth inhibition and cell death in mantle cell lymphoma

FEBS Lett. 2005 Dec 19;579(30):6885-9. doi: 10.1016/j.febslet.2005.11.020.

Abstract

We have earlier reported overexpression of the central and peripheral cannabinoid receptors CB1 and CB2 in mantle cell lymphoma (MCL), a B cell non-Hodgkin lymphoma. In this study, treatment with cannabinoid receptor ligands caused a decrease in viability of MCL cells, while control cells lacking CB1 were not affected. Interestingly, equipotent doses of the CB1 antagonist SR141716A and the CB1/CB2 agonist anandamide inflicted additive negative effects on viability. Moreover, treatment with the CB1/CB2 agonist Win-55,212-2 caused a decrease in long-term growth of MCL cells in culture. Induction of apoptosis, as measured by FACS/Annexin V-FITC, contributed to the growth suppressive effect of Win-55,212-2. Our data suggest that cannabinoid receptors may be considered as potential therapeutic targets in MCL.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arachidonic Acids / pharmacology
  • Benzoxazines
  • Biopsy
  • Breast Neoplasms / pathology
  • Cannabinoid Receptor Agonists
  • Cannabinoid Receptor Antagonists
  • Cannabinoids / pharmacology
  • Cell Death / drug effects*
  • Cell Division / drug effects*
  • Cell Line
  • Cell Line, Transformed
  • Cell Line, Tumor
  • Cell Transformation, Viral
  • Cells, Cultured
  • Dose-Response Relationship, Drug
  • Endocannabinoids
  • Female
  • Flow Cytometry
  • Humans
  • Leukemia, Plasma Cell / pathology
  • Ligands
  • Lymphoma, Mantle-Cell / pathology*
  • Mice
  • Morpholines / pharmacology
  • Naphthalenes / pharmacology
  • Piperidines / pharmacology
  • Polyunsaturated Alkamides
  • Pyrazoles / pharmacology
  • Receptors, Cannabinoid / classification
  • Receptors, Cannabinoid / metabolism*
  • Rimonabant

Substances

  • Arachidonic Acids
  • Benzoxazines
  • Cannabinoid Receptor Agonists
  • Cannabinoid Receptor Antagonists
  • Cannabinoids
  • Endocannabinoids
  • Ligands
  • Morpholines
  • Naphthalenes
  • Piperidines
  • Polyunsaturated Alkamides
  • Pyrazoles
  • Receptors, Cannabinoid
  • Win 55212-2
  • Rimonabant
  • anandamide