Bioassay-guided evaluation of anti-inflammatory and antinociceptive activities of pistachio, Pistacia vera L

J Ethnopharmacol. 2006 Apr 21;105(1-2):235-40. doi: 10.1016/j.jep.2005.10.023. Epub 2005 Dec 6.


The ethanolic and aqueous extracts prepared from different parts of Pistacia vera L. (Anacardiaceae) as well as its oleoresin were evaluated for their in vivo anti-inflammatory and antinociceptive activities. Among the extracts screened, only the oleoresin was shown to possess a marked anti-inflammatory activity against carrageenan-induced hind paw edema model in mice without inducing any gastric damage at both 250 and 500 mg/kg doses whereas the rest of the extracts were totally inactive. While the oleoresin was found to display significant antinociceptive activity at 500 mg/kg dose, the ethanolic and aqueous extracts belonging to fruit, leaf, branch and peduncle of Pistacia vera did not exhibit any noticeable antinociception in p-benzoquinone-induced abdominal contractions in mice. Fractionation of the oleoresin indicated the n-hexane fraction to be active, which further led to recognition of some monoterpenes, mainly alpha-pinene (77.5%) by capillary gas chromatography-mass spectrometry (GC-MS) as well as the oleoresin itself. alpha-Pinene was also assessed for its antinociceptive and anti-inflammatory activities in the same manner and exerted a moderate anti-inflammatory effect at 500 mg/kg dose.

MeSH terms

  • Analgesics / pharmacology*
  • Animals
  • Anti-Inflammatory Agents / pharmacology*
  • Drug Evaluation, Preclinical
  • Gas Chromatography-Mass Spectrometry
  • Male
  • Pistacia / chemistry*
  • Plant Extracts / pharmacology*
  • Rats


  • Analgesics
  • Anti-Inflammatory Agents
  • Plant Extracts