Comparison of the Properties of the Five Soluble Guanylyl Cyclase Subunits in Drosophila Melanogaster

J Insect Sci. 2005;5:12. doi: 10.1093/jis/5.1.12. Epub 2005 Apr 19.


The Drosophila melanogaster genome contains 5 genes that code for soluble guanylyl cyclase subunits. Two of these genes code for subunits, Gycalpha-99B and Gycbeta-100B, which form a conventional NO-sensitive guanylyl cyclase and the other three code for atypical subunits, Gyc-88E, Gyc-89Da and Gyc-89Db. The properties and distribution of Gyc-88E and Gyc-89Db have previously been described and here Gyc-89Da is described. Gyc-89Da only forms an active guanylyl cyclase when co-expressed with Gyc-88E. The three atypical subunits probably form two different heterodimers in vivo: Gyc-88E/89Da and Gyc-88E/89Db. Both of these heterodimers were slightly stimulated by NO donors and Gyc-88E/89Da showed a greater activation by Mn2+, with an increase in Vmax and a decrease in K(m), compared to Gyc-88E/89Db. Both Gyc-88E/89Da and Gyc-88E/89Db were expressed in neurons in both the peripheral and central nervous system. Although all three heterodimeric soluble guanylyl cyclases in D. melanogaster can be activated by NO and inhibited by ODQ, the atypical enzymes can be distinguished from the conventional soluble guanylyl cyclase by their sensitivity to the NO-independent activators YC-1 and BAY 41-2272, which will only activate the conventional enzyme.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • COS Cells
  • Chlorocebus aethiops
  • Cloning, Molecular / methods
  • DNA Primers / chemistry
  • Drosophila Proteins / biosynthesis
  • Drosophila Proteins / drug effects
  • Drosophila Proteins / genetics
  • Drosophila Proteins / physiology*
  • Drosophila melanogaster / enzymology*
  • Drosophila melanogaster / genetics
  • Drosophila melanogaster / growth & development
  • Ganglion Cysts / ultrastructure
  • Gene Expression Profiling
  • Gene Expression Regulation, Enzymologic*
  • Gene Order
  • Guanylate Cyclase / biosynthesis*
  • Guanylate Cyclase / drug effects
  • Guanylate Cyclase / genetics*
  • Guanylate Cyclase / physiology
  • In Situ Hybridization / methods
  • Life Cycle Stages / physiology
  • Nitric Oxide Donors / pharmacology
  • Protein Isoforms / chemistry
  • Protein Isoforms / genetics
  • Receptors, Cell Surface / biosynthesis
  • Receptors, Cell Surface / drug effects
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / physiology*
  • Reverse Transcriptase Polymerase Chain Reaction / methods
  • Time Factors


  • DNA Primers
  • Drosophila Proteins
  • Nitric Oxide Donors
  • Protein Isoforms
  • Receptors, Cell Surface
  • Guanylate Cyclase
  • Gyc76C protein, Drosophila