Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 37 (5), 299-306

Two Critical Factors Affecting the Release of Mitochondrial Cytochrome C as Revealed by Studies Using N,N'-dicyclohexylcarbodiimide as an Atypical Inducer of Permeability Transition

Affiliations

Two Critical Factors Affecting the Release of Mitochondrial Cytochrome C as Revealed by Studies Using N,N'-dicyclohexylcarbodiimide as an Atypical Inducer of Permeability Transition

Takenori Yamamoto et al. J Bioenerg Biomembr.

Abstract

N,N'-dicyclohexylcarbodiimide (DCCD) was earlier reported to have stimulatory effects on mitochondrial respiration and to induce mitochondrial swelling, when it was added to mitochondrial suspensions. These data seem to imply that DCCD caused the mitochondrial permeability transition (PT), but this possibility had never been investigated. In the present study, effects of DCCD on the mitochondrial structure and function were studied in detail. DCCD was found to induce mitochondrial PT in a cyclosporine A-insensitive manner. Electron microscopic analysis also supported the induction of the mitochondrial PT by DCCD. However, different from many other PT inducers, DCCD failed to cause massive release of mitochondrial cytochrome c. To understand the relationship between the induction of mitochondrial PT and the release of mitochondrial cytochrome c, we compared the actions of DCCD on mitochondrial structure and function with those of Ca2+, known as an ordinary PT inducer. As a result, two parameters considered to be critical for controlling the release of mitochondrial cytochrome c on the induction of PT were mitochondrial volume and the velocity of mitochondrial oxygen consumption.

Similar articles

See all similar articles

Cited by 5 articles

References

    1. FEBS Lett. 1997 Dec 1;418(3):282-6 - PubMed
    1. FEBS Lett. 1998 May 22;428(1-2):89-92 - PubMed
    1. J Bioenerg Biomembr. 1990 Oct;22(5):679-89 - PubMed
    1. Nature. 1999 Jun 3;399(6735):483-7 - PubMed
    1. Biochemistry. 1986 Mar 11;25(5):1015-21 - PubMed

Publication types

MeSH terms

LinkOut - more resources

Feedback