Acrylamide-induced oxidative stress and biochemical perturbations in rats

Toxicology. 2006 Feb 15;219(1-3):133-41. doi: 10.1016/j.tox.2005.11.008. Epub 2005 Dec 15.


Acrylamide is neurotoxic to experimental animals and humans. Also, it has mutagenic and carcinogenic effects. The present study was carried out to investigate the effects of different doses of acrylamide on some enzyme activities and lipid peroxidation in male rats. Animals were assigned at random to one of the following treatments: group 1 served as control, while groups 2, 3, 4, 5, 6 and 7 were treated with 0.5, 5, 25, 50, 250 and 500 microg/kg body weight of acrylamide, respectively in drinking water for 10 weeks. Acrylamide significantly decreased plasma protein levels and the activity of creatine kinase, while increased plasma phosphatases. The activities of transaminases and phosphatases were significantly decreased in liver and testes, while lactate dehydrogenase did not change compared to control group. Plasma and brain acetylcholinesterase activity was significantly decreased. The concentration of thiobarbituric acid reactive substances, and the activities of glutathione S-transferase and superoxide dismutase in plasma, liver, testes, brain, and kidney were increased in acrylamide-treated rats. On the other hand, results obtained showed that acrylamide significantly reduced the content of sulfhydryl groups and protein in different tissues. The present results showed that different doses of acrylamide exerted deterioration effects on enzyme activities and lipid peroxidation in a dose-dependent manner.

MeSH terms

  • Acrylamide / toxicity*
  • Animals
  • Blood Proteins / metabolism
  • Dose-Response Relationship, Drug
  • Enzymes / metabolism
  • Glutathione Transferase / metabolism
  • Lipid Peroxidation / drug effects
  • Liver / drug effects
  • Liver / enzymology
  • Male
  • Oxidative Stress / drug effects*
  • Rats
  • Rats, Sprague-Dawley
  • Sulfhydryl Compounds / metabolism
  • Superoxide Dismutase / metabolism
  • Thiobarbituric Acid Reactive Substances / metabolism
  • Tissue Distribution
  • Weight Gain / drug effects


  • Blood Proteins
  • Enzymes
  • Sulfhydryl Compounds
  • Thiobarbituric Acid Reactive Substances
  • Acrylamide
  • Superoxide Dismutase
  • Glutathione Transferase