A novel dual-axis iterative algorithm for electron tomography

J Struct Biol. 2006 Jan;153(1):55-63. doi: 10.1016/j.jsb.2005.10.005. Epub 2005 Nov 28.


A new algorithm for computing electron microscopy tomograms which combines iterative methods with dual-axis geometry is presented. Initial modelling using test data shows several improvements over both the weighted back-projection and simultaneous iterative reconstruction technique methods, with increased stability and tomogram fidelity under high-noise conditions. Preliminary experimental dual-axis reconstructions confirm the viability of the new algorithm.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Algorithms*
  • Fourier Analysis
  • Imaging, Three-Dimensional / methods
  • Microscopy, Electron / methods*
  • Tomography, X-Ray Computed / methods*