Transferable DHA-1 Cephalosporinase in Escherichia Coli

Int J Antimicrob Agents. 2006 Jan;27(1):77-80. doi: 10.1016/j.ijantimicag.2005.09.013. Epub 2005 Dec 13.

Abstract

Three Escherichia coli isolates resistant to third-generation cephalosporins but negative for extended-spectrum beta-lactamase production were isolated from hospitalised patients in Zagreb, Croatia, during June 2003 to February 2004. Resistance was due to the inducible production of a DHA-1 cephalosporinase. Each isolate contained an integron-associated bla(DHA-1)-ampR sequence carried by similar-sized plasmids, of which one was self-transferable. Serotyping and polymerase chain reaction typing using ERIC2 primer indicated that the isolates were distinct. This is the first description of DHA beta-lactamase production in E. coli.

MeSH terms

  • Bacterial Proteins / genetics
  • Cephalosporin Resistance / genetics*
  • Cephalosporinase / genetics*
  • Cephalosporins / pharmacology
  • Conjugation, Genetic
  • Croatia
  • Escherichia coli / drug effects
  • Escherichia coli / genetics*
  • Escherichia coli / isolation & purification
  • Escherichia coli Infections / microbiology
  • Escherichia coli Proteins / genetics*
  • Gene Transfer, Horizontal
  • Microbial Sensitivity Tests
  • Plasmids / genetics
  • Polymerase Chain Reaction / methods
  • beta-Lactams / pharmacology

Substances

  • Bacterial Proteins
  • Cephalosporins
  • Escherichia coli Proteins
  • beta-Lactams
  • AmpR protein, Bacteria
  • Cephalosporinase
  • DHA-1 cephalosporinase, E coli