Generation of DNA-free Escherichia coli cells by 2-aminopurine requires mismatch repair and nonmethylated DNA

J Bacteriol. 2006 Jan;188(1):339-42. doi: 10.1128/JB.188.1.339-342.2006.

Abstract

Undirected mismatch repair initiated by the incorporation of the base analog 2-aminopurine kills DNA-methylation-deficient Escherichia coli dam cells by DNA double-strand breakage. Subsequently, the chromosomal DNA is totally degraded, resulting in DNA-free cells.

MeSH terms

  • 2-Aminopurine / pharmacology*
  • Antimetabolites / pharmacology*
  • Base Pair Mismatch*
  • DNA Repair*
  • DNA, Bacterial / metabolism*
  • Escherichia coli / drug effects
  • Escherichia coli / genetics
  • Escherichia coli / growth & development*
  • Escherichia coli Proteins
  • Flow Cytometry
  • Site-Specific DNA-Methyltransferase (Adenine-Specific)

Substances

  • Antimetabolites
  • DNA, Bacterial
  • Escherichia coli Proteins
  • 2-Aminopurine
  • Site-Specific DNA-Methyltransferase (Adenine-Specific)
  • dam protein, E coli