A Low-Cost Open-Source SNP Genotyping Platform for Association Mapping Applications

Genome Biol. 2005;6(12):R105. doi: 10.1186/gb-2005-6-12-r105. Epub 2005 Dec 2.

Abstract

Association mapping aimed at identifying DNA polymorphisms that contribute to variation in complex traits entails genotyping a large number of single-nucleotide polymorphisms (SNPs) in a very large panel of individuals. Few technologies, however, provide inexpensive high-throughput genotyping. Here, we present an efficient approach developed specifically for genotyping large fixed panels of diploid individuals. The cost-effective, open-source nature of our methodology may make it particularly attractive to those working in nonmodel systems.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Chromosome Mapping / economics*
  • Chromosome Mapping / methods*
  • Cost-Benefit Analysis
  • Drosophila melanogaster / genetics
  • Genome, Insect / genetics
  • Genotype
  • Oligonucleotides / chemistry
  • Polymorphism, Single Nucleotide / genetics*
  • Sensitivity and Specificity

Substances

  • Oligonucleotides