The Protons of Gluconic Acid Are the Major Factor Responsible for the Dissolution of Tricalcium Phosphate by Burkholderia Cepacia CC-Al74

Bioresour Technol. 2006 May;97(7):957-60. doi: 10.1016/j.biortech.2005.02.017. Epub 2005 Dec 13.

Abstract

Burkholderia cepacia CC-Al74 with a high ability for solubilizing tricalcium phosphate (TCP) was used to study the P-solubilization mechanism. We collected filtrates able to solubilize TCP from the cultures of strain CC-Al74 and demonstrated that the P-solubilization increased from 0 microg ml(-1) to 200 microg ml(-1) during exponential growth, when the pH decreased from 8 to 3. HPLC-analysis revealed that the solubilization of TCP was mainly caused by the release of 16.3 mM gluconic acid. At this concentration, gluconic acid was capable of solubilizing 376 microg ml(-1) of TCP whereas water at pH 3 only solubilized 35 microg ml(-1). The difference is due to the final H+ concentrations which were 13.5 mM and 1 mM in 16.3 mM gluconic acid and deionized water, respectively at pH 3.

Publication types

  • Comparative Study

MeSH terms

  • Burkholderia cepacia / genetics
  • Burkholderia cepacia / growth & development
  • Burkholderia cepacia / metabolism*
  • Calcium Phosphates / metabolism*
  • Culture Media / chemistry
  • Culture Media / pharmacology
  • Filtration
  • Gluconates / analysis
  • Gluconates / chemistry*
  • Hydrochloric Acid / pharmacology
  • Hydrogen-Ion Concentration
  • Kinetics
  • Protons*
  • Solubility
  • Water / chemistry

Substances

  • Calcium Phosphates
  • Culture Media
  • Gluconates
  • Protons
  • Water
  • tricalcium phosphate
  • Hydrochloric Acid
  • gluconic acid