Construction and characterization of a series of vectors for Schizosaccharomyces pombe

Yeast. 2005 Dec;22(16):1307-14. doi: 10.1002/yea.1332.

Abstract

A set of vectors was created to allow cloning and expression studies in Schizosaccharomyces pombe. These vectors had a uniform backbone with an efficient Sz. pombe ARS, ARS3002, but different selectable markers--his3+, leu1+, ade6+ and ura4+. The vectors functioned efficiently as autonomously replicating plasmids that could also be converted into integrating vectors. The ura4+-containing vector was used to construct a Sz. pombe genomic library.

MeSH terms

  • Gene Library
  • Genetic Markers / genetics
  • Genetic Vectors / chemical synthesis
  • Genetic Vectors / genetics*
  • Plasmids / genetics
  • Schizosaccharomyces / genetics*

Substances

  • Genetic Markers