Prognostic significance of bcl-2 and p53 expression in colorectal carcinoma

J Zhejiang Univ Sci B. 2005 Dec;6(12):1163-9. doi: 10.1631/jzus.2005.B1163.


Objective: This study was designed to detect the expression of bcl-2 and p53 proteins in colorectal carcinomas and to determine their association with the patient survival and stage of the diseases.

Methods: Immunohistochemistry method was used to detect the expression of bcl-2 and p53 proteins in 93 cases of colorectal carcinoma. The stain results were obtained by analyzing the clinic-pathological characteristics of patients.

Results: Fifty-seven percent (53/93) of the colorectal carcinomas were bcl-2 protein positive. The positive rate of bcl-2 protein in lymph node involvement cases was lower (15/37) than the cases without node involvement (38/58, P<0.01). The positive rate of p53 protein was 43% (40/93) in colon-rectum carcinomas. No significant correlation was observed between p53 protein expression and clinic-pathological manifestations (P>0.05) but the survival was significantly worse (P=0.0001) in the p53 protein positive cases. Neither bcl-2 nor p53 alone was correlated with stage of the disease. When combined bcl-2/p53 status was analyzed, a group with bcl-2(+) and p53(-) had the best prognosis. This group was significantly associated with earlier Dukes' stages (P=0.1763). In multivariate Cox regression analysis, lymph node involvement and p53 protein expression were two independent factors correlated with survival time.

Conclusion: The expression of bcl-2 and p53 represent biological characteristics of colorectal carcinomas. Assessment of both bcl-2 and p53 status may be valuable in predicting the prognosis of patients.

Publication types

  • Clinical Trial

MeSH terms

  • Biomarkers, Tumor / metabolism*
  • China / epidemiology
  • Colorectal Neoplasms / diagnosis
  • Colorectal Neoplasms / metabolism*
  • Colorectal Neoplasms / mortality*
  • Female
  • Humans
  • Male
  • Middle Aged
  • Prevalence
  • Prognosis
  • Proto-Oncogene Proteins c-bcl-2 / metabolism*
  • Risk Assessment / methods*
  • Risk Factors
  • Survival Analysis
  • Survival Rate
  • Tumor Suppressor Protein p53 / metabolism*


  • Biomarkers, Tumor
  • Proto-Oncogene Proteins c-bcl-2
  • Tumor Suppressor Protein p53