Comparison of in vitro replication features of H7N3 influenza viruses from wild ducks and turkeys: potential implications for interspecies transmission

J Gen Virol. 2006 Jan;87(Pt 1):171-5. doi: 10.1099/vir.0.81187-0.

Abstract

In previous work, it was shown that turkey H7N3 influenza viruses, presumably derived 'in toto' from interspecies transmission of duck viruses in Northern Italy, had only 2 aa differences in haemagglutinin and a few amino acid differences as well as a 23 aa deletion in neuraminidase compared with duck viruses. Here, the replication of these duck and turkey viruses in Madin-Darby canine kidney cells was investigated with respect to virus-cell fusion and viral elution from red blood cells. Duck viruses showed similar receptor-binding properties to turkey viruses but possessed a higher pH of fusion activation than the turkey viruses. Conversely, turkey viruses were not able to elute from red blood cells. These data confirm that neuraminidase-stalk deletion impairs the release of virions from cells and also confirm existence of naturally occurring viruses with different pH fusion activities, raising the possibility that these features may play a role in the evolution of influenza viruses in different hosts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Wild / virology
  • Bird Diseases / transmission*
  • Bird Diseases / virology
  • Cell Line
  • Ducks
  • Hemagglutinin Glycoproteins, Influenza Virus / genetics*
  • Influenza A virus / genetics*
  • Influenza A virus / pathogenicity
  • Influenza in Birds / epidemiology*
  • Influenza in Birds / transmission*
  • Influenza in Birds / virology
  • Poultry Diseases / transmission*
  • Poultry Diseases / virology
  • Turkeys
  • Virus Replication*

Substances

  • Hemagglutinin Glycoproteins, Influenza Virus