The interphase microtubule cytoskeleton of five different phenotypes of microvessel endothelial cell cultures derived from bovine corpus luteum

Tissue Cell. 1992;24(3):347-54. doi: 10.1016/0040-8166(92)90051-8.

Abstract

The interphase microtubule cytoskeleton of five different microvessel endothelial cell cultures, recently established from bovine corpus luteum, was analysed using anti-tubulin immunofluorescence. An antibody against acetylated microtubules detected four cell types each of which possessed a single cilia. The length of the cilia were up to 10 microns for cell types 1 and 2. Ciliary stubs had a length of up to 0.37 microns in cell types 4 and 5. Cilia were missing in cell type 3. Long and short cilia were located in the perinuclear region from where cytoplasmic microtubules radiated. Cell type 3 displayed straight microtubules rather than the wavy path seen in the other cell types. The amount of tyrosinated microtubules visualized by a specific antibody was consistently higher than that of posttranslationally acetylated microtubules. The latter were more apparent in cell types 4 and 5 than in the other cell types. We conclude: Differences in the cytoplasmic microtubule inventory of each microvessel endothelial cell type points at individual functions maintained in culture.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylation
  • Animals
  • Antibodies, Monoclonal
  • Cattle
  • Cells, Cultured
  • Cilia
  • Corpus Luteum / blood supply*
  • Endothelium, Vascular / chemistry*
  • Female
  • Interphase
  • Microscopy, Fluorescence
  • Microtubules / chemistry*
  • Tyrosine

Substances

  • Antibodies, Monoclonal
  • Tyrosine