Cortactin is required for integrin-mediated cell spreading

Immunol Lett. 2006 Apr 15;104(1-2):124-30. doi: 10.1016/j.imlet.2005.11.011. Epub 2005 Dec 7.


Cortactin is an SH3 domain-containing protein that contributes to the formation of dynamic cortical actin-associated structures, such as lamellipodia and membrane ruffles. Here we show that expression of either the GFP-tagged N-terminal or the C-teminal halves of cortactin inhibits significantly the spreading of COS7 cells on fibronectin. Introducing inactivating point mutation into the SH3 domain of the C-terminal half of cortactin suspends the dominant negative effect of the construct. In addition, a vector-based RNA interference was used to knock-down endogenous level of cortactin in cells. We demonstrate that cortactin deficient cells were not able to spread. These results suggest that cortactin is required for integrin-mediated signalling pathways.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • COS Cells
  • Cell Adhesion
  • Cell Movement / genetics
  • Chlorocebus aethiops
  • Cortactin / analysis
  • Cortactin / genetics
  • Cortactin / physiology*
  • Fibronectins / metabolism
  • Green Fluorescent Proteins / analysis
  • Green Fluorescent Proteins / genetics
  • Integrins / metabolism*
  • Point Mutation
  • Protein Structure, Tertiary
  • RNA Interference
  • RNA, Small Interfering / genetics
  • RNA, Small Interfering / pharmacology
  • Recombinant Fusion Proteins / analysis
  • Recombinant Fusion Proteins / genetics
  • Transcriptional Activation


  • Cortactin
  • Fibronectins
  • Integrins
  • RNA, Small Interfering
  • Recombinant Fusion Proteins
  • Green Fluorescent Proteins