Lactobacillus helveticus fermented milk lowers blood pressure in hypertensive subjects in 24-h ambulatory blood pressure measurement

Am J Hypertens. 2005 Dec;18(12 Pt 1):1600-5. doi: 10.1016/j.amjhyper.2005.06.006.


Background: The present study was carried out to evaluate the blood pressure (BP)-lowering effect and the safety aspects of Lactobacillus helveticus LBK-16H fermented milk with high tripeptide doses on hypertensive subjects using 24-h ambulatory measurements (ABPM).

Methods: In a randomized, double blinded placebo-controlled parallel group study, 94 hypertensive patients not receiving any drug treatment were given 150 mL twice daily of either L. helveticus LBK-16H fermented milk with a high concentration of tripeptides (Ile-Pro-Pro 7.5 mg/100 g and Val-Pro-Pro 10 mg/100 g) or a control product, for 10 weeks after a 4-week run-in period. Twenty-four-hour ABPM were taken at the beginning and at the end of the intervention period. The average baseline systolic and diastolic BP values were 132.6 +/- 9.9/83.0 +/- 8.0 mm Hg in the L. helveticus group and 130.3 +/- 9.6 /80.2 +/- 7.0 mm Hg in the control group.

Results: There was a mean difference of -4.1 +/- 0.9 mm Hg in systolic (P = .001) and a -1.8 +/- 0.7 mm Hg in diastolic BP (P = .048) between the L. helveticus group and the control group. There was no difference in the sum of the adverse events (P = .820).

Conclusions: Lactobacillus helveticus LBK-16H fermented milk containing bioactive peptides, in daily use, does have a BP-lowering effect in hypertensive subjects and is thus a potential for the dietary treatment of hypertension.

Publication types

  • Comparative Study
  • Randomized Controlled Trial

MeSH terms

  • Analysis of Variance
  • Animals
  • Blood Pressure / drug effects
  • Blood Pressure Monitoring, Ambulatory
  • Circadian Rhythm
  • Double-Blind Method
  • Female
  • Fermentation
  • Humans
  • Hypertension / diet therapy*
  • Lactobacillus helveticus*
  • Male
  • Milk / chemistry
  • Milk / metabolism
  • Milk / microbiology*
  • Milk Proteins / administration & dosage
  • Milk Proteins / metabolism
  • Office Visits
  • Oligopeptides / administration & dosage


  • Milk Proteins
  • Oligopeptides
  • isoleucyl-prolyl-proline
  • valyl-prolyl-proline