Mass spectrometric identification of serine hydrolase OVCA2 in the medulloblastoma cell line DAOY

Cancer Lett. 2006 Sep 28;241(2):235-49. doi: 10.1016/j.canlet.2005.10.023. Epub 2005 Dec 20.


OVCA2 is a putative serine-hydrolase. Performing protein profiling in human tumour cell lines, OVCA2 was detected in DAOY medulloblastoma cells as a high abundance protein. The protein was unambiguously identified by 2D gel-electrophoresis and MALDI-MS and MS/MS, its presence was confirmed by western blotting. Immunohistochemistry revealed expression in medulloblastoma and predominantly in oligodendrocytes. Computational approaches predicted functional motifs and domains, interaction with apoptosis-related protein BAG and 3D structure. In addition to the presence of OVCA2 in medulloblastoma, it was furthermore detectable in three out of 10 human tumour cell-lines as a high abundance protein probably suggesting a role in the tumour biology.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Cell Line, Tumor
  • Cerebellar Neoplasms / enzymology*
  • Cerebellar Neoplasms / pathology
  • Electrophoresis, Gel, Two-Dimensional
  • Humans
  • Medulloblastoma / enzymology*
  • Medulloblastoma / pathology
  • Molecular Sequence Data
  • Peptide Mapping
  • Protein Processing, Post-Translational
  • Proteins / genetics
  • Proteins / metabolism*
  • Sequence Homology, Amino Acid
  • Serine Endopeptidases / genetics
  • Serine Endopeptidases / metabolism*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization*


  • OVCA2 protein, human
  • Proteins
  • Serine Endopeptidases