Artificial transcription factors increase production of recombinant antibodies in Chinese hamster ovary cells

Biotechnol Lett. 2006 Jan;28(1):9-15. doi: 10.1007/s10529-005-4680-7.


A randomized library that encodes for artificial zinc finger protein transcription factors (ZFP-TF) was constructed and screened for components that increased production of a monoclonal antibody (mAb-72) in Chinese hamster ovary (CHO) cells. One of these ZFP-TF, LK52, increased mAb-72 production approximately 10-fold at approximately 60% transduction efficiency; a mutated version of LK52, however, did not boost mAb-72 production. LK52 also increased production of other mAbs in CHO cells. These results demonstrate that ZFP-TF libraries can be used to identify components that improve antibody production in CHO cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / biosynthesis*
  • Antibodies, Monoclonal / genetics
  • CHO Cells
  • Cell Line
  • Cricetinae
  • Genetic Vectors
  • Humans
  • Peptide Library
  • Recombinant Proteins / biosynthesis
  • Retroviridae / genetics
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Transduction, Genetic
  • Zinc Fingers / genetics


  • Antibodies, Monoclonal
  • Peptide Library
  • Recombinant Proteins
  • Transcription Factors