Induction of RANTES and CCR5 through NF-kappaB activation via MAPK pathway in aged rat gingival tissues

Biotechnol Lett. 2006 Jan;28(1):17-23. doi: 10.1007/s10529-005-4681-6.

Abstract

Chemokine and chemokine receptor expression in gingival tissues plays a central role in periodontal disease during aging. In the present study, we explored the modulation of chemokines and chemokine receptors expression in aging rat gingival tissues. In the 24-month-old (Old) rat gingival tissues, RANTES and CCR5 mRNA and protein levels were 2-4 fold increased over those of the 6-month-old (Young) rats. The Old rats had considerable enhancement of all three of the studied MAPK activities: extracellular signal regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38 MAPK. These results suggest that age-related increases in RANTES and CCR5 expression are associated with increased IkappaBalpha, nuclear NF-kappaB, and MAPK activity in gingival tissues.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aging / physiology*
  • Analysis of Variance
  • Animals
  • Blotting, Western
  • Chemokine CCL5 / metabolism*
  • Electrophoretic Mobility Shift Assay
  • Gingiva / metabolism*
  • Male
  • Mitogen-Activated Protein Kinases / metabolism*
  • NF-kappa B / metabolism*
  • Proteins / analysis
  • RNA, Messenger / analysis
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, CCR5 / metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Chemokine CCL5
  • NF-kappa B
  • Proteins
  • RNA, Messenger
  • Receptors, CCR5
  • Mitogen-Activated Protein Kinases