Cytogenetic effect of technical glyphosate on cultivated bovine peripheral lymphocytes

Int J Hyg Environ Health. 2006 Jan;209(1):15-20. doi: 10.1016/j.ijheh.2005.07.005. Epub 2005 Aug 29.


A technical herbicide containing isopropyl amine salt of glyphosate was tested for induction of chromosome aberrations (CA) and sister chromatid exchanges (SCE) in cultured bovine peripheral lymphocytes. Cultures were exposed to a glyphosate formulation at concentrations ranging from 28 to 1120 micromol/l without and with metabolic activation. No clastogenic effect of the herbicide was found. Its genotoxic effect was confirmed in the SCE assay after 24 h of incubation. A statistically significant elevation in SCE induction was observed in each of the donors after application of the product at doses ranging from 56 to 1120 micromol/l. The highest concentrations (560 and 1120 micromol/l) also caused reduction of mitotic and proliferation indices. In the 2 h-assay with metabolic activation a statistically significant frequency of SCE was observed only in cultures treated with the agent at a concentration of 140 micromol/l.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Cell Proliferation
  • Cells, Cultured
  • Chromosome Aberrations / drug effects
  • Chromosomes, Mammalian / drug effects
  • Glycine / analogs & derivatives*
  • Glycine / toxicity
  • Lymphocytes / drug effects*
  • Mitotic Index
  • Sister Chromatid Exchange / drug effects


  • glyphosate, isopropyl amine salt
  • glyphosate
  • Glycine