Transcriptional regulation of the Neurospora circadian clock gene wc-1 affects the phase of circadian output

EMBO Rep. 2006 Feb;7(2):199-204. doi: 10.1038/sj.embor.7400595.

Abstract

WHITE COLLAR-1 (WC-1) is the limiting component of the White Collar Complex (WCC) controlling expression of the Neurospora circadian clock protein Frequency (FRQ). Accumulation of WC-1 is supported by FRQ on a post-transcriptional level. Here, we show that transcription of wc-1 is organized in a complex way. Three promoters drive transcription of wc-1. Pdist is dependent on WCC. Pprox is independent of WCC in darkness, but inducible by light in a WCC-dependent manner. A third promoter, Pint, is located in the wc-1 open reading frame and promotes expression of an amino-terminally truncated WC-1 isoform of unknown function. Expression of wc-1 by Pdist or Pprox alone, or by a heterologous promoter, affects the entrained phase of circadian conidiation and the response of Neurospora to light. Our results indicate that transcriptional regulation of wc-1 is required to modulate the circadian phase of clock output.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Biological Clocks
  • Blotting, Western
  • Circadian Rhythm / genetics*
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / physiology*
  • Feedback, Physiological
  • Fungal Proteins / chemistry
  • Fungal Proteins / genetics
  • Fungal Proteins / physiology*
  • Gene Expression Regulation, Fungal*
  • Luciferases / metabolism
  • Molecular Sequence Data
  • Neurospora / genetics*
  • Neurospora / physiology*
  • RNA, Fungal / analysis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transcription Factors / chemistry
  • Transcription Factors / genetics
  • Transcription Factors / physiology*
  • Transcription, Genetic*

Substances

  • DNA-Binding Proteins
  • FRQ protein, Neurospora crassa
  • Fungal Proteins
  • RNA, Fungal
  • Transcription Factors
  • wc-1 protein, Neurospora crassa
  • Luciferases