Arabidopsis KRPs have distinct inhibitory activity toward cyclin D2-associated kinases, including plant-specific B-type cyclin-dependent kinase

FEBS Lett. 2006 Jan 9;580(1):336-40. doi: 10.1016/j.febslet.2005.12.018. Epub 2005 Dec 19.


Arabidopsis contains seven Kip-related protein (KRP) genes encoding CDK (cyclin-dependent kinase) inhibitors (CKIs), which shares a restricted similarity with mammalian p27Kip1. Here, we analyze the characteristics of the KRPs. Although KRP1-KRP7 interact with active cyclin D2 (CYCD2)/CDKA and CYCD2/CDKB complexes to a similar extent, they inhibit kinase activity to a different extent. Our results suggest that inhibitory activity is related to the binding ability between KRP proteins and cyclin/CDK complexes, but secondary and tertiary structure may be also involved. These data provide the first evidence that KRPs inhibit kinase activity associated with plant-specific CDKB.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / metabolism*
  • Cyclin-Dependent Kinase Inhibitor Proteins / metabolism*
  • Cyclin-Dependent Kinase Inhibitor p27 / metabolism
  • Cyclin-Dependent Kinases / metabolism*
  • Cyclins / metabolism*
  • Multiprotein Complexes / metabolism*
  • Protein Structure, Quaternary
  • Protein Structure, Tertiary
  • Sequence Homology, Amino Acid


  • Arabidopsis Proteins
  • CycD2 protein, Arabidopsis
  • Cyclin-Dependent Kinase Inhibitor Proteins
  • Cyclins
  • Multiprotein Complexes
  • Cyclin-Dependent Kinase Inhibitor p27
  • CDKA1 protein, Arabidopsis
  • Cyclin-Dependent Kinases