Spatio-temporal patterns of intestine-specific transcription factor expression during postnatal mouse gut development

Gene Expr Patterns. 2006 Apr;6(4):426-32. doi: 10.1016/j.modgep.2005.09.003. Epub 2005 Dec 27.

Abstract

The small intestine matures from a primitive tube into morphologically and functionally distinct regions during gut development. Maximal expression of the genes encoding the digestive enzymes lactase-phlorizin hydrolase and sucrase-isomaltase is spatially restricted to distinct segments along the anterior-posterior axis of the small intestine and is temporally regulated during postnatal maturation. Transcription factors capable of interacting with the intestinal lactase and sucrase gene promoters are candidate regulators of spatio-temporal patterning during gut development and maturation. We aimed to quantitatively examine and compare the relative expression levels of a set of intestine-specific transcription factors along the anterior-posterior gut axis during postnatal maturation. Our analysis was focused on the transcription factors capable of regulating the intestinal lactase and sucrase-isomaltase genes. A real-time PCR protocol was used to quantitatively examine and compare spatially and temporally the relative transcript abundance levels for intestine-specific factors during postnatal intestinal maturation. Distinct spatial expressions patterns were detected along the length of the small intestine for PDX-1, Cdx-2, GATA-4, GATA-5, GATA-6, HNF-1alpha, HNF-1beta and CDP transcription factor genes. There is a general decline in transcript abundance for the factor genes during postnatal maturation. Defining the spatio-temporal expression patterns for intestine-specific transcription factor genes contributes to investigation of the roles that factor gradients play in mediating gut development and differentiation.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Body Patterning*
  • CDX2 Transcription Factor
  • GATA4 Transcription Factor / genetics
  • GATA4 Transcription Factor / metabolism
  • GATA5 Transcription Factor / genetics
  • GATA5 Transcription Factor / metabolism
  • GATA6 Transcription Factor / genetics
  • GATA6 Transcription Factor / metabolism
  • Gene Expression Regulation*
  • Hepatocyte Nuclear Factor 1-alpha / genetics
  • Hepatocyte Nuclear Factor 1-alpha / metabolism
  • Homeodomain Proteins / genetics
  • Homeodomain Proteins / metabolism
  • Intestine, Small / enzymology
  • Intestine, Small / growth & development*
  • Intestine, Small / metabolism*
  • Mice
  • Mice, Inbred BALB C
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism
  • RNA, Messenger / analysis
  • Repressor Proteins / genetics
  • Repressor Proteins / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sucrase-Isomaltase Complex / genetics
  • Sucrase-Isomaltase Complex / metabolism
  • Time Factors
  • Trans-Activators / genetics
  • Trans-Activators / metabolism
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*

Substances

  • CDX2 Transcription Factor
  • Cdx2 protein, mouse
  • Cux1 protein, mouse
  • GATA4 Transcription Factor
  • GATA5 Transcription Factor
  • GATA6 Transcription Factor
  • Gata4 protein, mouse
  • Gata5 protein, mouse
  • Gata6 protein, mouse
  • Hepatocyte Nuclear Factor 1-alpha
  • Homeodomain Proteins
  • Nuclear Proteins
  • RNA, Messenger
  • Repressor Proteins
  • Trans-Activators
  • Transcription Factors
  • pancreatic and duodenal homeobox 1 protein
  • Sucrase-Isomaltase Complex