Cloning and sequencing of the aldehyde oxidase gene from Methylobacillus sp. KY4400

Biosci Biotechnol Biochem. 2005 Dec;69(12):2435-8. doi: 10.1271/bbb.69.2435.

Abstract

The aldehyde oxidase genes (aods) from Methylobacillus sp. KY4400 were cloned, and sequenced. The sequences for small (aodS, 489 bp), medium (aodM, 993 bp), and large (aodL, 2,328 bp) subunit genes were determined. At least one additional ORF was indispensable for the expression of enzyme activity. The structural genes contained two [2Fe-2S] centers, an FAD binding site, and a molybdenum cofactor binding site.

MeSH terms

  • Aldehyde Oxidase / genetics*
  • Cloning, Molecular
  • DNA, Bacterial / biosynthesis
  • DNA, Bacterial / genetics
  • Databases, Genetic
  • Flavin-Adenine Dinucleotide / metabolism
  • Methylobacillus / enzymology*
  • Methylobacillus / genetics*
  • Molecular Weight
  • Molybdenum / metabolism
  • Sequence Analysis, Protein
  • Xanthine Oxidase / chemistry
  • Xanthine Oxidase / genetics

Substances

  • DNA, Bacterial
  • Flavin-Adenine Dinucleotide
  • Molybdenum
  • Xanthine Oxidase
  • Aldehyde Oxidase