Study on the antifertility effects of the plasmid DNA vaccine expressing partial brLDH-C4'

Reproduction. 2006 Jan;131(1):183-92. doi: 10.1530/rep.1.00262.


Partial cDNA sequence coding for Microtus brandti radde (Brandt's vole) testes-specific lactate dehydrogenase (brLDH-C4) was amplified by reverse transcription-polymerase chain reaction (RT-PCR). By inserting the product into the eukaryotic expression vector pCR3.1, pCR3.1-brLDH-C4' was obtained as the prototype of contraceptive DNA vaccine. Immunization with pCR3.1-brLDH-C4' in BALB/c mice generated antibodies specific to purified brLDH-C4' and native mouse LDH-C4 protein. The birth rate of the pCR3.1-brLDH-C4' immunized mice was found to be decreased significantly (80% lower than that of those immunized with pCR3.1). Functions of the elicited antibodies in sera from pCR3.1-brLDH-C4' inoculated mice were further explored. The results indicated that the antibodies from the mice injected with pCR3.1-brLDH-C4' could cause the agglutination of normal sperm suspension, while the ovarian structure and the development of ovarian follicles of these mice were not impaired, which gives a possible explanation for the immunocontraceptive effects of the pCR3.1-brLDH-C4' DNA vaccine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / isolation & purification
  • Antibodies, Monoclonal / pharmacology
  • Arvicolinae / genetics
  • Arvicolinae / immunology
  • Base Sequence
  • Bioreactors
  • Blotting, Western / methods
  • Contraception, Immunologic*
  • Enzyme-Linked Immunosorbent Assay / methods
  • Escherichia coli
  • Female
  • Fertility / drug effects*
  • Genetic Engineering
  • HeLa Cells
  • Humans
  • Immunization
  • Immunohistochemistry / methods
  • Isoenzymes / genetics
  • Isoenzymes / immunology
  • L-Lactate Dehydrogenase / genetics*
  • L-Lactate Dehydrogenase / immunology*
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Plasmids
  • RNA, Messenger / analysis
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / pharmacology
  • Sequence Alignment
  • Sperm Agglutination / drug effects
  • Transfection / methods
  • Vaccines, DNA / administration & dosage*
  • Vaccines, DNA / pharmacology


  • Antibodies, Monoclonal
  • Isoenzymes
  • RNA, Messenger
  • Recombinant Proteins
  • Vaccines, DNA
  • L-Lactate Dehydrogenase
  • lactate dehydrogenase C4