Derivation of human embryonic stem cells in defined conditions

Nat Biotechnol. 2006 Feb;24(2):185-7. doi: 10.1038/nbt1177. Epub 2006 Jan 1.


We have previously reported that high concentrations of basic fibroblast growth factor (bFGF) support feeder-independent growth of human embryonic stem (ES) cells, but those conditions included poorly defined serum and matrix components. Here we report feeder-independent human ES cell culture that includes protein components solely derived from recombinant sources or purified from human material. We describe the derivation of two new human ES cell lines in these defined culture conditions.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Cell Culture Techniques / methods*
  • Cell Differentiation
  • Cell Line
  • Cell Proliferation
  • Cell Survival
  • Culture Media / chemistry*
  • Culture Media / metabolism*
  • Humans
  • Stem Cells / cytology*
  • Stem Cells / physiology*
  • Tissue Engineering / methods*


  • Culture Media