Treatment with 1,25-dihydroxyvitamin D3 preserves glomerular slit diaphragm-associated protein expression in experimental glomerulonephritis

Int J Immunopathol Pharmacol. Oct-Dec 2005;18(4):779-90. doi: 10.1177/039463200501800422.

Abstract

In this study, we investigated the effect of 1,25(OH)2D3 on proteinuria and on the alteration of slit diaphragm-associated proteins induced by anti-Thy 1.1 in Wistar rats. Four groups of animals were studied: group I, anti-Thy 1.1 treated rats; group II, anti-Thy1.1 treated group that at day 2, after the onset of overt proteinuria, started the treatment with 1,25(OH)2D3; group III, normal control rats injected with vehicle alone; group IV, rats that received only 1,25(OH)2D3. At day 2, in group I and II, before the administration of 1,25(OH)2D3, protein excretion was significantly increased when compared to controls. Overt proteinuria was maintained until day 14 in group I whereas in group II protein excretion was significantly reduced from day 3 to day 14. Moreover, treatment with 1,25(OH)2D3 abrogated podocytes injury, detected as desmin expression and loss of nephrin and zonula occludens-1 (ZO-1), two slit diaphragm-associated proteins, and glomerular polyanion staining, that were observed in group I. In conclusion, these results suggest that 1,25(OH)2D3 administrated with a therapeutic regiment may revert proteinuria, counteracting glomerular podocyte injury.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcitriol / therapeutic use*
  • Desmin / biosynthesis
  • Female
  • Fluorescent Antibody Technique
  • Glomerulonephritis, Membranoproliferative / drug therapy*
  • Glomerulonephritis, Membranoproliferative / metabolism*
  • Glomerulonephritis, Membranoproliferative / pathology
  • Kidney Glomerulus / drug effects*
  • Kidney Glomerulus / metabolism*
  • Kidney Glomerulus / pathology
  • Membrane Proteins / biosynthesis
  • Phosphoproteins / biosynthesis
  • Polymers / metabolism
  • Proteinuria / metabolism
  • Rats
  • Rats, Wistar
  • Tissue Fixation
  • Vitamins / therapeutic use*
  • Zonula Occludens-1 Protein

Substances

  • Desmin
  • Membrane Proteins
  • Phosphoproteins
  • Polymers
  • Tjp1 protein, rat
  • Vitamins
  • Zonula Occludens-1 Protein
  • nephrin
  • polyanions
  • Calcitriol