Intein-mediated in vitro synthesis of lipidated Ras proteins

Chem Commun (Camb). 2006 Jan 21;(3):260-2. doi: 10.1039/b511736d. Epub 2005 Nov 28.

Abstract

Fully functional lipid-modified Ras proteins suitable for the study of Ras-membrane interactions and embodying exclusively native amide bonds can be synthesized in preparative amounts by means of Expressed Protein Ligation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amides / chemistry
  • Binding Sites
  • Inteins / physiology*
  • Lipids / chemistry*
  • Lipoproteins / chemical synthesis*
  • Membrane Proteins / chemistry
  • Models, Chemical
  • Protein Engineering / methods*
  • Protein Interaction Mapping / methods*
  • ras Proteins / chemistry*

Substances

  • Amides
  • Lipids
  • Lipoproteins
  • Membrane Proteins
  • ras Proteins